Latent Herpes Simplex Virus Infection of Sensory Neurons Alters Neuronal Gene Expression

HveC is abundantly expressed in neurons and can block viral entry in several neuronlike cell lines (66). Our results confirm that recombinant virus vaccines expressing cytokine genes can enhance the overall protection against infection, with the IL-12p35 vaccine being the most efficacious of those tested. ICP27 binds to RNA, with a preference for G/C-rich sequences (6), and directly interacts with viral mRNA in infected cells (4). This work was also supported by BBSRC grant BB/J014419/1, Medical Research Council grant G0701185, Wellcome Trust project grant WT089111MA, and collaborative grant BelVir from Belspo. At various time points, the extracellular viruses were removed by proteinase K and … The increased EGFP mRNA half-life, and therefore silencing suppression, that was observed in cells infected by viruses defective in either vhs or ICP27 function compared to that observed in wild-type-virus-infected cells indicates that both host shutoff functions contribute significantly to the overall stability of the EGFP target mRNA in infected cells compared to results for mock-infected cells (Table 2).

(10) that input genomes are circularized rather than joined end to end. 7C). Cells were washed twice with RPMI 1640 medium (Sigma-Aldrich) supplemented as described above, counted, and placed (3 × 106) in FACScan tubes (Sarstedt, Verona, Italy). Cell identification was based on morphology and on differential immunostainings for neurons (anti-MAP2 detection) and glial cells (anti-GFAP staining). Intraneoplastic administration of the TK-deleted strains induced tumor regression in several animal tumor models, with severe necrosis of the tumor tissue.130, 131, 132, 133, 134 However, in tumor models in severe combined immunodeficient (SCID) mice, these mutants were shown to spread into normal tissues,135 indicating a lack of tumor specificity. We have developed a virus-based gene delivery procedure to construct an expression system for the investigation of receptor-ligand interactions in the VNO.

Taken together, the data suggest that ICP4 interacts with components of TFIID and Mediator in the context of viral infection, and this may explain the broad transactivation properties of ICP4. Two-way immunoprecipitation experiments revealed that the membrane protein UL20, which is found as a protein complex with gK, interacted with gM while gM did not interact with other viral glycoproteins. Furthermore, HSV‐1 is being engineered for use as a gene therapy vector to convey genes from the periphery to the central nervous system or for direct infection of cerebral and other tumors, resulting in infection and destruction of tumor cells but not of normal brain tissue [4]. The increases in UL42 expression were always much more substantial than any effect on ICP4 in both ATRX- and hDaxx-depleted cells. Viruses.HSV-2 strains 186TKΔKpn (9) and 186syn+ (29) and HSV-1 strain KOS were kindly provided by David Knipe (Harvard Medical School). The box plots show the distribution of simulated breakpoints generated by the Monte Carlo process in each of the three region types.

The space between envelope and vacuolar membranes is filled with a dense substance (Fig. We did not specifically account for this as this is an evolving area of research. These two mechanisms make specific predictions about the levels of expression of particular cellular genes in latently infected versus uninfected neurons. Furthermore, HSV-1 US3 cooperates with glycoprotein B to rapidly inhibit CD1d antigen presentation and natural killer T-cell activation (23). The mean central channel diameter in HSV-1-infected nuclei, however, was 92 nm. HSV-1 remained responsive to the IE gene transcriptional inducer hexamethylene bisacetamide (HMBA), and to transcriptional transactivation by simultaneous co-infection of the ganglion compartment (GC) with helper virus.

These results will be discussed in the context of how replication proteins are recruited to replication forks. The IAP family proteins such as XIAP, cIAP1, and cIAP2 have been shown to bind caspases to block their activation [ 31 ]. The complex structure of HSV-2 gD bound to nectin-1.To elucidate the molecular basis of the nectin-1 recognition by HSV-2 gD, complex crystals were prepared and the structure was successfully solved at 3.2-Å resolution. Although work by Leege and colleagues suggests that deletion of gM does not impact the nuclear egress of both PRV and HSV-1 capsids (48), a compensatory mechanism cannot be ruled out in light of the built-in functional redundancy commonly observed in herpesviruses. This led us to investigate the interaction of ICP22 and p53 in HSV-1-infected cells and the role(s) of p53 in viral replication in the presence and absence of ICP22. Samples were analyzed using fluorescence microscopy (Zeiss, Germany).

The virus pellets were resuspended in sterile PBS and divided into aliquots. While HSV-1 can cause both oral and genital infections, HSV-2 is transmitted almost exclusively sexually and is the major cause of genital herpes. Infection of vaccinia virus (VV) in mouse embryonic fibroblasts (MEFs) sensitizes the cells to TNF-α-induced necrosis (7). To monitor VICE domains, the cells were stained for both Hsc70 and the IE protein ICP4, the latter being a marker for viral RCs (22). The long C-terminal loop (loops 270–306), as shown in Figure 5c, spreads over the gD/nectin-1 interaction interface. (i) During latency, LAT+ viruses caused significantly more functional exhaustion of total and HSV-1-specific TG resident CD8+ T cells than LAT− viruses.

Selective detection of viral immediate-early, early, and delayed-early gene expression in CJ83193-infected cells.Studies with replication-defective HSV-1 mutant viruses elegantly documented that the degree of humoral and cell-mediated immunity induced by replication-defective viruses correlates directly with the extent of viral gene expression occurring in infected cells (41). The patient improved within 48 h and signed out of the hospital against medical advice on day 4. Erythrocytes were removed by 5-min exposure to ACK (150 mM NH4Cl, 0.1 mM EDTA, 10 mM KHCO3; pH 7.3) lysing buffer. (2006) New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells. Together with previous results (23), this suggests that envelope proteins gE, gG, gI, gJ, gM, UL45p, and Us9p are individually dispensable for entry into two cell lines that support endocytic entry. The γ134.5 protein facilitates viral replication as well as spread via amino-terminal sequences in vivo.To evaluate γ134.5 in vivo, we investigated viral replication in a mouse ocular infection model.

Moreover, there is a possibility that the 2-h incubation at 4°C followed by a shift up to 37°C allows for sufficient initial membrane fusion and/or pore formation resulting in exposure of surface-bound remaining viral particles and gB to NMHC-IIA and NMHC-IIB. We hypothesized that tetherin overexpression might saturate any anti-tetherin activities mediated by the virus and reveal tetherin sensitivity. Suarez Summer Research Fellowships.

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